Industry-leading fidelity confirmed by pyrosequencing. Published error rates of Taq, Taq-based long-range PCR blends, and proofreading DNA polymerases (Q5, New England Biolabs and Phusion®, Thermo Scientific) are compared to the error rate of KAPA HiFi HotStart DNA Polymerase (1 error per 3.54 x 10-6 nucleotides incorporated), which was confirmed by deep sequencing on the 454 platform, and is is 50 – 100 times lower than that of Taq.
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